Objective:The aim of this study is to investigate the underlying mechanisms of action of Mongolian Mind-Body Interactive Therapy(MMBIT)in patients with insomnia through whole transcriptome sequencing.Methods:A total of 5 patients with chronic insomnia were subjected to intervention treatment using MMBIT.Peripheral blood samples were collected before and after the intervention treatment for high-throughput transcriptome sequencing.By comparing and analyzing the changes in transcriptomic data before and after treatment,we aim to establish a solid foundation for uncovering the mechanisms of action of MMBIT in treating chronic insomnia.Results:Significant clinical improvements in sleep were observed in patients with chronic insomnia before and after receiving MMBIT,as assessed by the Athens Insomnia Scale,Pittsburgh Sleep Quality Index,and Insomnia Severity Index(P ≤0.05).Whole transcriptome analysis revealed 838 differentially expressed mRNAs after treatment,with 425 downregulated and 413 upregulated mRNAs.Additionally,2,445 differentially expressed lncRNAs were identified,with 1,211 downregulated and 1,234 upregulated lncRNAs.Moreover,29 differentially expressed miRNAs were detected,including 12 upregulated miRNAs and 17 downregulated miRNAs.Significantly different mRNAs were found to be significantly enriched in pathways associated with cytokines.Further analysis of the differentially expressed genes in this pathway using the ceRNA network revealed an interaction network involving 11 miRNAs,23 lncRNAs,and 23 mRNA nodes.Conclusion:This study revealed that MMBIT intervention led to a reduction in the inflammatory cytokine IL17b in patients with chronic insomnia.Additionally,significant changes were observed in the expression of LOC285889,COL18A1-AS,hsa-miR-1249-3p,and hsa-miR-1908-5p,which are associated with IL17b.Further investigation with an expanded sample size is warranted to explore these findings in greater detail.
维普
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