Isolation,Identification and Biological Characteristics of Fruit Rot Pathogen of Yellow Passion Fruit in Fujian Province
In order to explore the pathogen causing postharvest fruit rot of yellow passion fruit in Fujian prov-ince and its biological characteristics,the pathogen was isolated from fruit.Its pathogenicity was measured by Koch's postulates,and the pathogenic morphological characteristics were observed.The pathogen was identi-fied by homology and phylogenetic analysis combined with molecular biological identification.Influences of dif-ferent temperature,pH value,carbon source and nitrogen source on the mycelia growth of fruit rot pathogen were preliminarily studied.Results showed that,according to the morphological and phylogenetic identifica-tion,the pathogen causing fruit rot of yellow passion fruit in Fujian Province was Lasiodiplodia theobromae.Moreover,the optimum temperature,pH value,carbon source and nitrogen source for the hyphal growth of L.theobromae were 28 ℃,pH 6,glucose and potassium nitrate,respectively.
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