首页|福建黄果西番莲果腐病菌分离鉴定及其生物学特性

福建黄果西番莲果腐病菌分离鉴定及其生物学特性

Isolation,Identification and Biological Characteristics of Fruit Rot Pathogen of Yellow Passion Fruit in Fujian Province

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为探究引起福建黄果西番莲采后果腐病的病原菌及其生物学特性,组织分离病果病原菌,采用柯赫氏法验证其致病性,观察病菌形态特征,结合分子生物学鉴定,采用同源性分析与系统发育树构建鉴定病原菌,并初步研究不同温度、pH值、碳源、氮源等对病原菌菌落生长的影响.结果表明,根据形态学和分子生物学鉴定,引起福建黄果西番莲采后果腐病的病原菌为可可毛色二孢Lasiodiplodia theobromae,28 ℃、pH值6、葡萄糖、硝酸钾分别是L.theobromae菌落生长最适温度、pH值、碳源和氮源.
In order to explore the pathogen causing postharvest fruit rot of yellow passion fruit in Fujian prov-ince and its biological characteristics,the pathogen was isolated from fruit.Its pathogenicity was measured by Koch's postulates,and the pathogenic morphological characteristics were observed.The pathogen was identi-fied by homology and phylogenetic analysis combined with molecular biological identification.Influences of dif-ferent temperature,pH value,carbon source and nitrogen source on the mycelia growth of fruit rot pathogen were preliminarily studied.Results showed that,according to the morphological and phylogenetic identifica-tion,the pathogen causing fruit rot of yellow passion fruit in Fujian Province was Lasiodiplodia theobromae.Moreover,the optimum temperature,pH value,carbon source and nitrogen source for the hyphal growth of L.theobromae were 28 ℃,pH 6,glucose and potassium nitrate,respectively.

yellow passion fruitfruit rotLasiodiplodia theobromaepH valuecarbon sourcenitrogen source

林育钊、陈月莉、蒋璇靓、郑金水、陈洪彬

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泉州师范学院海洋与食品学院,福建泉州,362000

福建省泉州市农业科学研究所,福建泉州,362212

黄果西番莲 果腐病 Lasiodiplodia theobromae pH值 碳源 氮源

福建省自然科学基金福建省自然科学基金

2023J019022021J01976

2024

中国南方果树
中国农业科学院柑桔研究所

中国南方果树

CSTPCD北大核心
影响因子:0.527
ISSN:1007-1431
年,卷(期):2024.53(1)
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