Study of in vitro Regeneration Technology for Actinidia arguta Qiyimei No.6
Using the non-bud stem segment as explants for in vitro culture has the advantages of low cost,convenient materials,and abundant test materials.However,previous studies have shown that the induced re-generation rate of non-bud stem of Actinidia arguta was extremely low.In this experiment,the non-bud stem segment of A.arguta Qiyimei No.6 was used as explants to explore the effects of different plant growth regu-lators,light intensity,temperature and seedling age on adventitious bud induction.The results showed that the best explants were non-bud stem segment of 30-day seedling.The best medium for induction of adventi-tious buds from A.arguta under 20 ℃ of room temperature,12 h of daylight and 50 lx of light intensity was MS+2 mg·L-1 ZT.The adventitious buds generated in an indirect way.After 40 days of culture,calli in light-green color began to form at both ends of the non-bud stem segment,and red spots appeared on the sur-face of calli and began to form adventitious buds on the 45th day.The callus induction rate,callus differentia-tion rate and the number of adventitious buds reached the maximum at 55th day,at 100％,100％and 18.87/piece,respectively.When the seedlings reached about 3 cm,they were transferred to MS+0.4 mg·L-1 IBA for rooting culture.After 30 days of culture,the rooting rate,number of roots,root length and root diameter reached 100％,8.53 per plant,1.68 cm and 0.14 cm,respectively.
万方数据
