Study on the Shoot Tip Tissue Culture System and Its Effect on Elimi-nation of Virus for Strawberry Cultivar Fenyu No.1
Fenyu No.1 is a new early-maturing,disease-resistant pink fruit strawberry variety derived through cross-breeding.With the shoot tip of Fenyu No.1 strawberry plants cultivated in the greenhouse as the test material,the virus-free and rapid propagation system of Fenyu No.1 strawberry was established by explant disinfection,shoot tip stripping,adventitious bud induction and rooting culture.Detection of strawberry crin-kle virus(SCV),strawberry mottle virus(SMoV),strawberry mild yellow edge virus(SMYEV)and straw-berry vein banding virus(SVBV)in shoot tip cultured seedlings and greenhouse plants were conducted with PCR.The results showed that the best sterilization treatment of Fenyu No.1 strawberry shoot tip was 75％alcohol for 30 s,then 0.1％mercury chloride for 10 min.Separation of stolon bud usually needs to peel off one tender leaf and seven young leaves from stolon.MS with 0.5 mg·L-1 6-BA,MS with 0.1 mg·L-1 6-BA was the optimum medium for initial culture and subculture,respectively.The suitable rooting medium is 1/2 MS without plant growth regulators.was detected in samples cultivated in greenhouse.SVBV was eliminated by shoot tip tissue cultured,and SCV,SMoV and SMYEV were not detected in virus-free plants.The shoot tip virus-free and rapid propagation system established in this study will provide a useful reference for the in-dustrialized seedling breeding of Fenyu No.1 strawberry.
Fenyu No.1strawberryshoot tip tissue culturestrawberry virus
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