Effects of different carbon and nitrogen ratios on nutrient content and bacterial community changes in compost made from Morella rubra branches
In order to study the effects of different C/N ratios on the nutrient,humus components and bacterial community structure in the process of fermentation and composting of Morella rubra branches,C/N ratios of 30∶1,60∶1 and 90∶1 were set for compost of Morella rubra branches to determine the nutrient and humus components,to compare the relative abundance of bacteria,analyze the Alpha and Beta diversity of bacterial community and the correlation of compost nutrient factors to bacterial community by 16S rRNA Illumina Miseq high-throughput sequencing of bacterial community.The results showed that the contents of total nitrogen(TN),total phosphorus(TP)and total potassium(TK)decreased with the increase of C/N ratio,while the contents of organic matter(OM)and humin(HM)increased with the increase of C/N ratio.The contents of TN,TK,humic acid(HA)and fulvic acid(FA)increased significantly with the extend of fermentation time,while the contents of OM and HM decreased significantly with the extend of fermentation time(p＜0.05).C/N ratio was extremely significantly correlated with OM and TN(p＜0.01).The relative abundance of Firmi-cutes and Actinobacteria bacteria decreased with the increase of C/N ratio,while the relative abundance of Pro-teobacteria and Bacteroides bacteria increased with the increase of C/N ratio.In the high temperature stage,the relative abundance of Firmicutes and Actinobacteria bacteria was significantly higher than that of other sta-ges.The abundance and diversity of bacteria communities in different C/N treatments were significantly differ-ent.The C/N ratio played a decisive role in the bacterial community structure of the compost pile,and the TN,OM and temperature also had certain effects on the bacterial community structure of the compost pile.There was a significant correlation between bacterial community structure and C/N ratio,TN and OM,which showed that C/N ratio played a key role in the nutrient content and bacterial community distribution in com-post of Morella rubra branches.
compostC/N ratioMorella rubraorganic matterhumusrelative abundance of bacteria
万方数据
维普
