Research on soil enzyme activity and fungal community structure in rhizosphere soil of Actinidia chinensis Planch with root rot
Study of fungal community structure in rhizosphere soil of Actinidia chinensis Planch infected and non-infected by root rot disease can provide a theoretical basis for studying the occurrence mechanism and con-trol of kiwifruit root rot.Samples of rhizosphere soil of 9-year-old kiwifruit tree infected and non-infected by kiwifruit root rot disease were collected in Hongyang kiwifruit orchards in Jietou Town,Tiantai County,Zhe-jiang Province to analyze the differences between fungal community structure and diversity index,and determine the activities of soil urease,sucrase and acid phosphatase by high throughput sequencing techniques.Results showed that the activities of soil urease,sucrase,phosephatase in rhizosphere soil of Actinidia chinensis Planch infected by root rot disease were extremely significantly inhibited(p＜0.01),and the Chao1 indexes of fungal were significantly lower that of non-infected plants(p＜0.05).At phylum level,the average relative abun-dance of some dominant fungal in rhizosphere soil of root rot disease infected kiwifruit plants were extremely significantly lower than those of non-infected plants(p＜0.01),such as Basidiomycota,Fungi-unclassified and Zygomycota,while the average relative abundance of Ascomycota in rhizosphere soil of infected kiwifruit plants were extremely significantly higher than that of non-infected plants(p＜0.01).At genus level,the increased relative abundance of fungal in rhizosphere soil of infected kiwifruit plants including Gibberella,Nectria,Fu-sarium,Pseudogymnoascus and Hypocrea with extreme significance(p＜0.01),while the decreased relative a-bundance of fungal in rhizosphere soil of infected kiwifruit plants including Agaricomycetes-unclassified,Cla-dorrhinum,Trechispora,Ophiocordyceps,Acremonium,Ascobolus,Trichoderma and Mortierella with extreme significance(p＜0.01).After root rot infection,the microflora changed at the phylum and genus level of rhizo-sphere soil fungi and broke the original microbial antagonistic balance.
kiwifruit root rothigh throughput sequencingfungal community structureactivity of soil enzyme
万方数据
维普
