Kiwifruit is a dioecious fruit that originated in China.By developing functional markers associated with the sex determination of kiwifruit,the sex of hybrid offspring or germplasm resources at the seedling stage could be detected,which greatly improved the efficiency of early sex trait selection,thus laying a founda-tion for germplasm resource innovation in an economical and efficient manner.Primers were designed based on the conserved exon sequences of female suppress gene SyGl and male improve gene FrBy in the sex determina-tion non-recombinant region of Y chromosome of kiwifruit,the effectiveness was proven by different popula-tion materials,and the high efficiency primer pair was screened.Effect of different molecular markers was compared by using agarose gel electrophoresis,target fragment sequencing and fluorescence capillary electro-phoresis methods.By introducing internal reference ITS primers,multiple PCR system and fluorescence capil-lary electrophoresis detection system were established,and the effect of the detection system was tested by 389 samples.The results showed that two sex-specific markers were obtained including Ms1 and Ms2,which could be used for sex identification of kiwifruit.Introduction of internal reference ITS primer decreased the ex-periment error caused by DNA quality.Three hundred and eighty-nine samples including varieties of Actinidia chinensis Planch.,A.deliciosa and wild germplasm resources such as A.valvata,A.eriantha,A.glauco-phylla and A.carnosifolia var.glaucescens were tested by multiple PCR system(ITS,Ms1,Ms2)and fluo-rescence capillary electrophoresis detection system.Sex of 370 samples was accurately identified,with accura-cy rate of 95.11%.The sex detection system developed in this study could improve the identification efficiency of sex marker,is suitable for molecular marker assisted selection of sex traits in kiwifruit.
维普
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