首页|3株生防菌对猕猴桃软腐病的作用

3株生防菌对猕猴桃软腐病的作用

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为探究生防菌对猕猴桃软腐病的作用,选用前期获得的 3 株生防菌(贝莱斯芽孢杆菌 P2-1、耐盐芽孢杆菌PL7 和多黏芽孢杆菌 NL4)进行室内抑菌试验、猕猴桃软腐病接种和自然发病防治试验,并研究了 3 株生防菌对猕猴桃果实病程相关基因表达的影响.试验结果表明,菌株 P2-1、PL7 和 NL4 对猕猴桃软腐病菌(Botryosphaeria dothidea)菌丝生长的抑制率分别为 83.49%、74.82%和 49.33%,P2-1 的抑制效果最好,PL7 次之,NL4 最差;3 株生防菌对接种猕猴桃软腐病均具有抑制作用,其中以P2-1 的效果最好,PL7 次之,NL4 最差;3 株生防菌均能显著降低猕猴桃软腐病自然发病率,其中以P2-1 处理的发病率最低;3 株生防菌处理均对猕猴桃果实品质无影响;3 株生防菌处理猕猴桃果实后,果实中基因PR1、PR5 和SOD的表达显著上调.研究结果可为菌株P2-1、PL7 和 NL4 开发为防治猕猴桃软腐病的生物制剂提供参考.
Effect of three strains of biocontrol bacteria on kiwifruit soft rot
In order to explore the effect of biocontrol bacteria on kiwifruit soft rot,three strains(Bacillus velezensis,B.halotolerans and Paenibacillus polymyxa)of biocontrol bacteria obtained in the previous study were used for inhibition tests,control experiments on kiwifruit soft rot under inoculation and natural condi-tions.The effects of these strains on expression levels of pathogenesis-related genes in kiwifruit fruit were studied.The results showed that the inhibition rate of P2-1,PL7 and NL4 strain on mycelial growth of Botry-osphaeria dothidea was 83.49%,74.82%,and 49.33%,respectively.The inhibitory effect of P2-1 was the best,followed by PL7,and NL4 was the worst.Three strains of biocontrol bacteria had inhibitory effects on the inoculated kiwifruit soft rot,among which P2-1 had the best effect,followed by PL7,and NL4 had the lowest effect.All the biocontrol strains could significantly reduce the natural incidence rate of kiwifruit soft rot,and the incidence rate of P2-1 treatment was the lowest.The biocontrol bacteria treatment had no effect on the quality of kiwifruit fruit.The biocontrol bacteria treatment significantly enhanced the expression levels of genes(PR1,MdPR5 and SOD)in kiwifruit fruit.These results could provide reference for the development of P2-1,PL7 and NL4 as biological agents for control kiwifruit soft rot.

kiwifruit soft rotBotryosphaeria dothideabiocontrol bacteriaBacillus velezensisB.halotol-eransPaenibacillus polymyxa

王丽、侯珲、涂洪涛、黄天祥、柳友亮、袁洪波

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中国农业科学院郑州果树研究所,郑州,450009

中国农业科学院中原研究中心,河南新乡,453003

猕猴桃软腐病 葡萄座腔菌 生防菌 贝莱斯芽孢杆菌 耐盐芽孢杆菌 多黏芽孢杆菌

河南省重点研发专项中国农业科学院科技创新工程中央级公益性科研院所基本科研业务费专项

221111111800CAAS-ASTIP-2023-ZFRI-031610192023105

2024

10.13938/j.issn.1007-1431.20230244

中国南方果树
中国农业科学院柑桔研究所

中国南方果树

CSTPCD北大核心
影响因子：0.527
ISSN：1007-1431
年,卷(期)：2024.53(3)
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