Screening of antagonistic actinomycetes against citrus pathogens and analysis of their active substances
To screen strains for the prevention and control of citrus diseases,the most harmful pathogen to post-harvest citrus fruits,Penicillium italicum,was used as an indicator pathogen.An antagonistic actinomy-cete was isolated from soil using a plate gradient dilution method.The strain was identified through morpho-logical observation and 16S rDNA sequencing analysis,and stability analysis of the antibacterial active sub-stances produced by the strain was further conducted.The results showed that a strain of Streptomyces globo-sus tz13 was selected from the soil with an inhibition zone diameter of 16.69±1.74 mm to Penicillium itali-cum.The antibacterial substances of strain tz13 had strong tolerance to temperature,ultraviolet t light,acid,and weak alkali.Additionally,it displayed inhibitory effects on another important post-harvest pathogen of cit-rus fruits:Penicillium digitatum,as well as other plant pathogenic fungi(such as Phytophthora capsici,Fusarrium oxysporum f.sp.niveum,Rhizoctonia solani and Phytophthora parasitica var.nicotianae).These findings indicated that strain tzl3 had commercial development value in control of citrus diseases.
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