番石榴花芽分化形态结构及内源激素动态变化研究
Study on the morphological structure of flower bud differentiation and the dynamic changes of endogenous hormones in Psidium guajava L.
黄婉莉 1张朝坤 1张冬敏 1郑诚乐2
作者信息
- 1. 漳州市农业科学研究所,福建漳州,363005
- 2. 福建农林大学园艺学院,福州,350002
- 折叠
摘要
为揭示番石榴花芽分化过程中内源激素的变化规律,以"红香1号"番石榴为试材,应用石蜡切片技术对花芽进行解剖观察,采用HPLC-MS/MS法测定花芽分化过程中其内源激素含量.结果表明,根据番石榴花器官发育特征将其花芽分化形态建成过程划分为始分化期、花萼原基分化期、花瓣原基分化期、雄蕊原基分化期、雌蕊原基分化期和雌蕊雄蕊成熟期6个时期.随着花芽分化进程,花芽吲哚乙酸和赤霉素含量变化规律相似,呈逐渐下降趋势;脱落酸和玉米素核苷含量总体呈先上升后下降的趋势,在花瓣原基分化期两者含量均达到最高,且显著高于其他时期.花芽分化过程中,脱落酸/赤霉素、脱落酸/吲哚乙酸、玉米素核苷/赤霉素和玉米素核苷/吲哚乙酸均呈先上升后下降的趋势,在花芽形态始分化期最低,在花瓣原基分化期最高,显著高于其他时期.推测低水平的花芽吲哚乙酸和赤霉素含量有利于番石榴花芽分化,高水平脱落酸和玉米素核苷含量有利于花芽分化,脱落酸/赤霉素、脱落酸/吲哚乙酸、玉米素核苷/赤霉素和玉米素核苷/吲哚乙酸升高与花芽分化诱导有关,促使番石榴向生殖生长转变.
Abstract
In order to reveal the changes of endogenous hormones in the process of flower bud differentiation of guava,Hongxiang No.1 guava was used as experimental material,and the flower buds were dissected and ob-served by paraffin section technology.The content of endogenous hormones in the process of flower bud differ-entiation was determined by HPLC-MS/MS.The results showed that according to the development character-istics of guava flower organ,the process of flower bud differentiation and morphogenesis was divided into six stages:initial differentiation,sepal primordium differentiation,petal primordium differentiation,stamen pri-mordium differentiation,pistil primordium differentiation,and pistil and stamen maturation.With the process of flower bud differentiation,the change regulations of endogenous hormones IAA and GA3 were similar,showing a gradual downward trend.The contents of ABA and TZR increased first and then decreased,and both reached the highest level at the stage of petal primordium differentiation,which were significantly higher than those of at other stages.During flower bud differentiation,the ratios of endogenous hormones ABA/GA3,ABA/IAA,TZR/GA3 and TZR/IAA all increased first and then decreased,with the lowest levels in the early stage of flower bud morphological differentiation and the highest levels in the stage of petal primordi-um differentiation,which were significantly higher than those of in other stages.It was inferred that low levels of IAA and GA3,and high levels of ABA and TZR,are conducive to flower bud differentiation of guava.The increase of ABA/GA3,ABA/IAA,TZR/GA3 and TZR/IAA ratio is related to induction of flower bud differ-entiation,which promotes the transformation of guava to reproductive growth.
关键词
番石榴/花芽分化/解剖结构/脱落酸/赤霉素/吲哚乙酸Key words
Psidium guajava L./flower bud differentiation/anatomical structure/ABA/GA3/IAA引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据