Seed dormancy and germination are precisely regulated by various factors such as endogenous hor-mones and external environment.Clean water soaking and low temperature stratification treatment are often used in production to break the dormancy process and promote germination of pear seeds.To analyze the mo-lecular mechanism of dormancy release in pear seeds,Cuiguan pear seeds were soaked in clean water for 6 hours and then stored in sand under low-temperature for 60 days to accelerate the germination.The ABA con-tent of pear seeds at 17 stages of this process was detected.The ABA content showed a decreasing trend dur-ing the seed soaking process and 7 days of low-temperature sand storage and then remained stable.Samples in seven key periods were selected for RNA sequencing.The results of GO analysis showed that differentially ex-pressed genes were assigned into 40 functional groups.The results of KEGG analysis showed that differential-ly expressed genes were mainly enriched in five pathways,including ribosome,microbial metabolism in diverse environments,carbon metabolism,biosynthesis of cofactors,and biosynthesis of amino acids.A co-expression network was constructed using transcriptome and ABA content data.The results showed that expressions of 11 genes(Pbr009501,Pbr016256,Pbr011612,Pbr033750,Pbr015393,Pbr000885,Pbr037755,Pbr039726,Pbr020341,Pbr003677 and Pbr015873)were highly correlated with ABA content,with correla-tion coefficients ranging from 0.91 to 0.96.These results provide important information for exploring the key genes that regulate ABA levels during pear seed dormancy and germination processes.
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