Analysis of key genes associated with ABA pathway related to the stratification process of Cuiguan pear seed by transcriptome sequencing
Seed dormancy and germination are precisely regulated by various factors such as endogenous hor-mones and external environment.Clean water soaking and low temperature stratification treatment are often used in production to break the dormancy process and promote germination of pear seeds.To analyze the mo-lecular mechanism of dormancy release in pear seeds,Cuiguan pear seeds were soaked in clean water for 6 hours and then stored in sand under low-temperature for 60 days to accelerate the germination.The ABA con-tent of pear seeds at 17 stages of this process was detected.The ABA content showed a decreasing trend dur-ing the seed soaking process and 7 days of low-temperature sand storage and then remained stable.Samples in seven key periods were selected for RNA sequencing.The results of GO analysis showed that differentially ex-pressed genes were assigned into 40 functional groups.The results of KEGG analysis showed that differential-ly expressed genes were mainly enriched in five pathways,including ribosome,microbial metabolism in diverse environments,carbon metabolism,biosynthesis of cofactors,and biosynthesis of amino acids.A co-expression network was constructed using transcriptome and ABA content data.The results showed that expressions of 11 genes(Pbr009501,Pbr016256,Pbr011612,Pbr033750,Pbr015393,Pbr000885,Pbr037755,Pbr039726,Pbr020341,Pbr003677 and Pbr015873)were highly correlated with ABA content,with correla-tion coefficients ranging from 0.91 to 0.96.These results provide important information for exploring the key genes that regulate ABA levels during pear seed dormancy and germination processes.
NETL
NSTL
万方数据
