猕猴桃9个种41份种质花粉形态观察与分析
Observation and analysis of pollen morphology of 41 germplasm of 9 species of kiwifruit
何利钦 1王丽华 1庄启国 1张瑶 1王凤琪 1张茜1
作者信息
- 1. 四川省自然资源科学研究院/中国-新西兰猕猴桃"一带一路"联合实验室,成都,610015
- 折叠
摘要
为了解猕猴桃属植物间的亲缘关系,以9个猕猴桃种41份种质的花粉为供试材料,采用扫描电镜结合ImageJ图像处理软件对花粉进行形态学观测,并根据花粉极轴长度(P)、赤道轴长度(E)、萌发沟长度、萌发沟宽度、极轴长度/赤道轴长度(P/E)和大小(P×E)指标对供试材料进行聚类分析.结果表明,供试花粉均为单粒花粉,均以单粒、等极和辐射对称形式存在.极轴长度范围在21.50～33.40μm之间,赤道轴长度范围在9.87～16.24 μm之间,萌发沟长度范围在17.53～29.08μm之间,萌发沟脊宽度范围在0.47～2.03μm之间,花粉粒大小(极轴×赤道轴)范围在218.68～543.18 μm2之间,极轴长度/赤道轴长度范围为1.36～2.26,花粉形状为超长球形或长球形,萌发孔类型均为三孔沟,极面观为三裂圆形,均为N3P4C5型.外壁纹饰分为波纹状、颗粒状和疣状,占样品总数的比例分别为63.41％、19.51％和17.08％.在遗传距离为7.5时,供试材料可以分为3个类群;在遗传距离为1.5时,供试材料可以分为6个类群,其中,中华猕猴桃被分为了 4类.在两个遗传距离时,四萼猕猴桃均单独分为一类.
Abstract
To understand the genetic relationship within kiwifruit genus,pollens of forty-one germplasm of 9 kiwifruit species were used as test materials,and the morphology of pollen was observed by scanning electron microscopy combined with ImageJ.The tested materials were cluster analyzed according to six trait indicators of pollen including polar axis length(P),equatorial axis length(E),germination ditch length,germination ditch width,the ratio of P to E and size.The results showed that the test pollens were all single-grain pollen,and in single-grain,isopolar and radially symmetric forms.The polar axis lengths ranged from 21.50 to 33.40μm,and the equatorial axis lengths ranged from 9.87 to 16.24 μm,the germination ditch lengths ranged from 17.53 to 29.08 μm,the germination ditch widths ranged from 0.47 to 2.03 μm,pollen grain size(polar axis × equatorial axis)ranged from 218.68 to 543.18 μm2,the ratio of polar axis length to equatorial axis length ranged from 1.36 to 2.26.The shape of the pollens was perprolate or prolate,and the germination pore types were all triple pore grooves.The polar surface view was triple cleft round,all of which were N3P4C5 type.The three types of outer wall ornamentation were corrugated,granular and warty,accounting for 63.41％,19.51％and 17.08％of the total number of samples,respectively.At a genetic distance of 7.5,the test mate-rials can be divided into 3 taxa.At a genetic distance of 1.5,the test materials could be divided into 6 taxa,a-mong which,Actinidia chinensis Planch.was divided into 4 taxa.At those two genetic distances,Actinidia tetramera Maxim.was divide into one taxon alone.
关键词
猕猴桃/花粉形态/扫描电镜/聚类分析Key words
kiwifruit/pollen morphology/scanning electron microscopy/cluster analysis引用本文复制引用
基金项目
四川省科技计划(2021YFYZ0023)
四川省科技计划(2020ZYD042)
四川省省级科研院所基本科研业务费(2021JDKY0023)
出版年
2024
国家科技期刊平台
NSTL
万方数据