Identification of kiwifruit canker pathogen in Shiyan City and its control
Kiwifruit bacterial canker caused by Pseudomonas syringae pv.actinidiae(Psa)seriously influ-ences the growth of kiwifruit plants in many areas.To determine the pathogen morphology and biovars in northwest of Hubei Province,and screen the effective fungicides,explore the control effect of rain-shelter culti-vation,so as to provide scientific basis for control the disease,the pathogen and biovar were identified through morphological and molecular biology techniques.The inhibition zone method was used to test the inhibitory effect of 15 pesticides on Psa.The incidence rate,disease index and control effect of Psa were investigated and compared under rain-shelter and outdoor cultivation.The results showed that the strain K-2 was identified as Psa and biovar 3 using the morphological characteristics and 16S rDNA and specific primer.Seven pesticides had inhibition effect on Psa by indoor toxicity test.The control effect of rain-shelter cultivation on Psa was 58.06％,and reached 83.87％with application of 50％Chloroisobromine-cyanuric-acid SP under rain-shelter cultivation,which indicated that the rain-shelter cultivation had better control effect on kiwifruit bacterial canker.
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