Establishment of in vitro rapid propagation system for Malus hupehensis
As an excellent apple rootstock,Malus hupehensis is widely used in apple production.In order to further improve the efficiency of in vitro rapid propagation of M.hupehensis,the spring stem segments with buds of M.hupehensis were used as explants to explore the effects of different explants disinfection methods as well as different hormone ratios on the proliferation and rooting of tissue cultured seedlings.The results showed that the explants immersed in 75％alcohol for 30 seconds,and then in 2％sodium hypochlorite for 12 min,had the best disinfection effect,with a survival rate of 81.11％.The optimal proliferation medium was MS+1.0 mg/L 6-BA+0.1 mg/L IBA,with a proliferation coefficient of 7.09.In addition,the optimal rooting medium was 1/2 MS+0.15 mg/L NAA with rooting rate of 75.55％,and the average number and length of roots were 3.23 and 3.57 cm,respectively.The survival rate of the transplanted tissue culture seed-lings was 90％in substrate of vermiculite:perlite:nutrient soil=1∶1∶1.The in vitro rapid propaga-tion system provided technical support for the year round cultivation of M.hupehensis rootstock seedlings.
NETL
NSTL
万方数据
