基于银纳米簇比色传感器快速检测水果维生素C的方法
Rapid determination of vitamin C in fruit by a silver nanocluster-based colorimetric sensor
杨海波 1周鹏程 1孟利峰 1赵龙龙1
作者信息
- 1. 山西农业大学果树研究所,山西晋中,030801
- 折叠
摘要
为提升水果品质分析效率,建立了基于银纳米簇比色传感器快速检测水果维生素C的方法.该方法以偏磷酸溶液萃取水果中的维生素C,以石墨化炭黑吸附干扰物质.以硝酸银水溶液和聚甲基丙烯酸钠水溶液混合加热搅拌4 h制备聚甲基丙烯酸修饰的银纳米簇材料,银纳米簇为球形,银纳米簇的平均直径为1 nm,加入维生素C以后,维生素C还原银离子从而增大了银纳米簇的尺寸并改变了银纳米簇溶液的光学性能,通过溶液颜色在感观上深浅变化和RGB色彩模式下蓝色值变化可实现维生素C含量的裸眼半定量分析和手机定量分析.在优化得到最佳检测条件后,结果表明,该检测方法可检出的维生素C最小含量为0.03 mg/kg,可定量的维生素C最小含量为0.1 mg/kg;该检测方法对葡萄糖、果糖、蔗糖、钙离子、锌离子、钾离子、叶酸等物质表现出了较好的抗干扰能力;该检测方法成功应用于橙、葡萄和梨果实维生素C的分析.将银纳米簇材料应用于水果的品质分析,有助于加快检测速度,降低检测成本,实现水果的精准快速分级.
Abstract
In order to enhance the efficiency of fruit quality analysis,a colorimetric sensor based on silver nanoclusters was established for the rapid detection of vitamin C in fruits.The vitamin C was extracted from fruit by metaphosphoric acid solution,and the interfering substances in fruit were absorbed by graphitized car-bon black.Polymethacrylic acid-modified silver nanocluster material was prepared by mixing and heating an a-queous solution of silver nitrate and sodium poly methacrylate for 4 hours.These silver nanoclusters were spherical with an average diameter of 1 nm.Silver ions were reduced by vitamin C to increase the size of silver nanocluster and change the optical properties of silver nanocluster solution.The vitamin C content can be semi-quantitatively analyzed by visual inspection based on the color change of the solution and quantitatively analyzed using a mobile phone by measuring changes of the blue value in the RGB color mode.After optimizing the de-tection conditions,the results showed that the limit of detection and quantitation content of vitamin C was 0.03 and 0.1 mg/kg,respectively.The method showed good anti-interference ability against substances such as glucose,fructose,sucrose,calcium ions,zinc ions,potassium ions,and folic acid.The method was success-fully applied to the analysis of vitamin C in oranges,grapes,and pears.The application of the silver nanoclus-ter material in fruit quality analysis is helpful to accelerate the detection speed,reduce the detection cost,and realize the accurate and rapid sorting of fruit.
关键词
维生素C/水果/银纳米簇/传感器/比色法Key words
vitamin C/fruit/silver nanocluster/sensor/colorimetric method引用本文复制引用
基金项目
山西省现代农业产业技术体系建设专项(2023CYJSTX07-18)
出版年
2024
国家科技期刊平台
NSTL
万方数据