Rapid determination of vitamin C in fruit by a silver nanocluster-based colorimetric sensor
In order to enhance the efficiency of fruit quality analysis,a colorimetric sensor based on silver nanoclusters was established for the rapid detection of vitamin C in fruits.The vitamin C was extracted from fruit by metaphosphoric acid solution,and the interfering substances in fruit were absorbed by graphitized car-bon black.Polymethacrylic acid-modified silver nanocluster material was prepared by mixing and heating an a-queous solution of silver nitrate and sodium poly methacrylate for 4 hours.These silver nanoclusters were spherical with an average diameter of 1 nm.Silver ions were reduced by vitamin C to increase the size of silver nanocluster and change the optical properties of silver nanocluster solution.The vitamin C content can be semi-quantitatively analyzed by visual inspection based on the color change of the solution and quantitatively analyzed using a mobile phone by measuring changes of the blue value in the RGB color mode.After optimizing the de-tection conditions,the results showed that the limit of detection and quantitation content of vitamin C was 0.03 and 0.1 mg/kg,respectively.The method showed good anti-interference ability against substances such as glucose,fructose,sucrose,calcium ions,zinc ions,potassium ions,and folic acid.The method was success-fully applied to the analysis of vitamin C in oranges,grapes,and pears.The application of the silver nanoclus-ter material in fruit quality analysis is helpful to accelerate the detection speed,reduce the detection cost,and realize the accurate and rapid sorting of fruit.
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