Physiological responses of eleven lemon rootstock germplasms to waterlogging stress and their waterlogging tolerance evaluation
Exploring the physiological response and tolerance of different rootstock germplasm plants to waterlogging stress could provide theoretical basis for the breeding of waterlogging tolerant lemon rootstock and the study of waterlogging resistance mechanisms.One-year-old seedlings of eleven rootstocks were used as experimental material,normal water management(control)and consistent flooding treatment were set up.The physiological response of the tested germplasms to waterlogging stress was studied by measuring relative conductivity,the content of chlorophyll,soluble protein,soluble sugar,and malondialdehyde,and catalase activity.The waterlogging tolerance of each material was further evaluated by principal component analysis and membership function method.Finally,the rootstocks were clustered according to the comprehensive evaluation value.The results showed that after waterlogging stress treatment,the relative conductivity of different rootstocks increased,the contents of chlorophyll and soluble protein decreased,and the contents of soluble sugar,malondialdehyde and hydrogen peroxide activity increased or decreased in different materials.Among the tested germplasms,the waterlogging tolerance of Bailimeng No.1,Suancheng and Xiangcheng was very strong,and that of Taiguoxiangyuan and Bailimeng 2 was stronger,followed by Daguohonglimeng,Suanyou,and Xiaoqingning with moderate waterlogging tolerance.Hongningmeng and Cuningmeng were not tolerant to waterlogging.
lemonrootstockwaterlogging toleranceprincipal component analysismembership function methodcomprehensive evaluation