Induction and in vitro regeneration of Siraitia grosvenorii tubers
In order to meet the needs of germplasm conservation as well as the production of excellent seedlings of Siraitia grosvenorii,stem segment with buds of Bolin No.2 S.grosvenorii was used as explant,and MS medium with different concentrations of naphthalene acetic acid(NAA),6-benzyl adenine(6-BA)and paclobutrazol was used to induce tuber and for in vitro preservation and growth recovery,and then transplanting.The tuber induction rate,survival rate,plant height and number of leaves under different treatments were compared.The results showed that the optimal medium for tuber induction and in vitro preservation of the test sample of S.grosvenorii was 1/2 MS+0.05 mg/L NAA+0.50 mg/L 6-BA+1.00 mg/L paclobutrazol,with the tuber induction rate of 97.67％,and survival rate of 87.00％after 540 days of in vitro preservation.The optimal medium for induced tuber growth recovery was MS+0.5 mg/L NAA+1.00 mg/L 6-BA,with the survival rate of 99.00％,showing good growth of roots and adventitious buds.The transplanting experiment revealed that the survival rate of the induced tubers reached 97.33％,higher than that of tissue culture seedlings,with the best plant growth.There was no significant difference in the transplanting performance between the tissue culture seedlings of stem segment culture from growth recovered inducing tuber and conventional tissue culture seedlings,both of them exhibited good growth.
万方数据
维普
