Study on fruit sugar and organic acid characteristics in different apple germplasm resources
Analyzing the composition of fruit sugar and acid,content characteristics of different apple varieties,and screening the evaluation index of sugar and acid quality can provide reference for evaluation of sugar and acid quality of apple varieties and breeding of excellent new varieties.Six approved apple varieties including Huashuo,Micui,Meiwei,Yanfu No.8,Qincui and Qinmi,and 14 cross bred superior varieties introduced in Weining County of Guizhou Province were used as materials to determine the normal quality and the components and contents of sugar and acid in fruits by HPLC,to screen the evaluation indicators of sugar and acid quality by correlation analysis and principal components analysis,and cluster the different varieties(lines)by K-mean clustering method.The results showed that the single fruit weight of the 20 varieties(lines)ranged from 139.68 g to 352.50 g.Among them,the single fruit weight and longitudinal and transverse diameters of 19-147 were the largest,and the single fruit weight,longitudinal diameter and peeled hardness of 16-112 were the smallest.The content of total soluble sugar ranged from 70.70 mg/g to 135.84 mg/g.The proportion of fructose in total soluble sugar was 46.38%-65.50%,and the contents of sucrose and glucose were lower than that of fructose,followed by sorbitol.Malic acid accounted for more than 59.03%of the total acid content,with the highest proportion of 80.67%,followed by succinic acid,citric acid,oxalic acid,quinic acid and ascorbic acid in turn.Four indicators,including total soluble sugar,sweetness value,malic acid and sweet sour ratio,were screened as the sugar and acid quality evaluation indicators of apple varieties(lines).Based on the sugar and acid quality evaluation indicators,the 20 varieties(lines)were clustered into four categories,including high sugar and high acid,high sugar and low acid,low sugar and high acid,and low sugar and low acid.
applegermplasm resourcessugar and acid componentsquality evaluation