Cloning and expression analysis of transcription factor LcZAT12 in litchi
To investigate the role of LcZAT12 in the development and maturation of litchi fruits,the full-length sequence of LcZAT12 was cloned from Heli and its late-maturing bud mutant strains MS1 and MS2,and bioinformatics analysis was performed.By using real-time fluorescence quantitative technology,the ex-pression patterns of LcZAT12 in different tissues and organs of litchi,as well as in the peel and seed during fruit development were analyzed.The results showed that the total length of LcZAT12 is 429 bp,and encodes a protein with 142 amino acids.The protein properties of LcZAT12 was predicted using online websites.It was found that the protein is located in the nucleus,and the phosphorylation is mainly serine,without signal peptides or transmembrane domains.The secondary structure of the protein is mainly Alpha helix and Random coil.The amino acid homologous sequence alignment results showed that LcZAT protein contained two highly conserved domains.The phylogenetic tree analysis revealed that the LcZAT12 protein had the closest relation-ship with homologous proteins of Acer saccharum and A.yangbiense.The spatiotemporal expression pattern suggested that the LcZAT12 protein may play a crucial role in the development of litchi fruits.
万方数据
维普
