In order to accurately detect Xanthomonas citri pv.citri,reduce false positive and false negative detection results,and provide reference for quarantine identification for agricultural law enforcement depart-ments and ports,14 pairs of conventional PCR and real-time fluorescence PCR primers and probes were used for comparison analysis of the detection specificity and sensitivity of 22 strains of X.citr i pv.citri and 9 other strains of Xanthomonas from different countries and regions.The selected primers were tested and verified by 9 samples with suspected symptoms of citrus canker.The results showed that the primer pairs of JYF5(XccF05)/JYR5(XccR05)and Xac01/Xac02 had higher specificity,and the detection sensitivity of both to X.citri pv.citri DNA were 3 pg/μL.The other 12 pairs of primers and probes showed different degree of false positive or false negative.Among them,XAcF/XAcR was false negative for Citrus aurantifolia samples and positive strains from Thailand.Based on the above results,JYF5(XccF05)/JYR5(XccR05)and Xac01/Xac02 were recommended for the detection of X.citri pv.citri.
维普
万方数据