Screening of primers for PCR detection of Xanthomonas citri pv.citri
In order to accurately detect Xanthomonas citri pv.citri,reduce false positive and false negative detection results,and provide reference for quarantine identification for agricultural law enforcement depart-ments and ports,14 pairs of conventional PCR and real-time fluorescence PCR primers and probes were used for comparison analysis of the detection specificity and sensitivity of 22 strains of X.citr i pv.citri and 9 other strains of Xanthomonas from different countries and regions.The selected primers were tested and verified by 9 samples with suspected symptoms of citrus canker.The results showed that the primer pairs of JYF5(XccF05)/JYR5(XccR05)and Xac01/Xac02 had higher specificity,and the detection sensitivity of both to X.citri pv.citri DNA were 3 pg/μL.The other 12 pairs of primers and probes showed different degree of false positive or false negative.Among them,XAcF/XAcR was false negative for Citrus aurantifolia samples and positive strains from Thailand.Based on the above results,JYF5(XccF05)/JYR5(XccR05)and Xac01/Xac02 were recommended for the detection of X.citri pv.citri.
万方数据
维普
