Dof(DNA binding with one finger)基因家族在植物的生长发育和响应逆境胁迫过程中发挥着重要作用.为了解番木瓜中Dof基因家族成员信息,通过生物信息学手段对番木瓜全基因组中Dof基因家族进行鉴定,分析其理化性质、基因结构、保守基序、系统发育、顺式作用元件以及在乙烯处理下的转录谱,并利用实时荧光定量PCR技术(RT-qPCR)分析Dof基因在番木瓜幼苗中响应红蓝光的表达模式.结果表明,番木瓜基因组中共鉴定出CpDofs 20个,它们分布在18条染色体上,氨基酸数230～499个,蛋白理论分子量23.88～53.56 kD,且均位于细胞核中.系统进化分析表明,20个CpDofs蛋白被分为3个亚家族,即ClassⅠ含2个,Class Ⅱ含6个,Class Ⅱ含12个.MEME分析发现所有CpDofs蛋白都含有Motif 1,推测Motif 1是CpDofs家族成员的重要组成部分.顺式作用元件分析发现,CpDofs的启动子区域存在大量光响应元件,而qRT-PCR分析发现,红光和蓝光能够诱导CpDofs基因表达.以上结果对研究CpDofs功能及响应红光和蓝光调控机制奠定了基础.
Genome-wide identification of the Dof gene family in Carica papaya and its expres-sion analysis under different lights treatment
The Dof(DNA binding with one finger)gene family plays an important role in plant growth and de-velopment,and response to adverse stresses.In order to understand the information of Dof gene family mem-bers in Carica papaya,the Dof gene family in the whole genome of C.papaya was identified by bioinformat-ics,and its physicochemical properties,gene structure,conserved motifs,phylogeny,cis-acting elements,and transcriptional profiles under ethylene treatment were analyzed.The expression patterns of Dof genes in re-sponse to red and blue light in C.papaya seedlings were analyzed by real-time fluorescence quantitative PCR(RT-qPCR).The results showed that 20 CpDofs were identified from C.papaya genome,which were dis-tributed on 18 chromosomes.The number of amino acids of encoded proteins ranging from 230 to 499,with theoretical molecular weights from 23.88 to 53.56 kD,and all of them located in the nucleus.Phylogenetic e-volution analysis showed that these 20 CpDof proteins were classified into three subfamilies,including Class Ⅰ(2),Class Ⅱ(6),and Class Ⅲ(12).The MEME analysis revealed that all CpDof proteins contained Motif 1,which was presumed to be an important component of CpDof family members.In addition,cis-acting element analysis revealed the presence of a large number of light-responsive elements in the promoter region of CpDofs,while RT-qPCR analysis showed that red and blue lights could induce the expression of CpDof genes.The a-bove results laid the foundation for the study of the function of CpDofs and the regulatory mechanism in re-sponse to red and blue lights.
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