ISSR classification of 23 characteristic germplasm resources of banana
To clarify the genetic relationship of banana germplasms and provide a theoretical basis for the sub-sequent research on crossbreeding of bananas for high yield and disease resistance,ISSR molecular marker a-nalysis and genetic relationships of 23 banana germplasm(mainly wild and local banana germplasm)preserved in the banana germplasm resource nursery of South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences were carried out and analyzed.The DNA of young leaves of 23 banana germ-plasms was extracted.Selected ISSR primers were used for molecular markers screening and genetic diversity analysis.The results showed that twelve primers were screened from forty ISSR primers.A total of 126 clear and reproducible bands were amplified from 23 germplasms,and all the bands were polymorphic.Among which,UBC807,UBC812 and UBC820 amplified the largest number of bands of 13,while UBC821 produced the least number of bands of 6.The results of cluster analysis showed that the 23 banana germplasms could be classified into eight groups.The first group included Bengal plantain,Zhengguo wild plantain and South Asian finger plantain.The second group included Tall recluse plantain,Nujiang wild plantain 5,Zhiman pink plan-tain,Nujiang wild plantain 6 and Pseudogolden Finger.The third group included Tianbao 988,Golden Finger,Red River dwarf plantain and Hainan red plantain.The fourth group included Baoshan pink plantain,Taiwan No.8,Zengcheng wild plantain,Xinglong red plantain and Lemu wild plantain.The fifth group included pur-ple stemmed plantain and Baoshan wild plantain.The sixth group included Baoshan wild plantain 1 and purple flower wild banana.The seventh group included Shanbasho 1 and the eighth group was Zhongshan Longya plantain.Zhongshan Longya banana had a low similarity coefficient and a distant genetic relationship with oth-er 22 banana materials.Among other groups,the similarity coefficients were high,the genetic relationship was close,and the genetic background was similar.The screened 12 ISSR primers could effectively classify these 23 banana germplasm resources.
万方数据
维普
