Identification of genes related to dehiscent fruit in jujube based on comparison between Muzao and its fission-resistant variation line V2
The fruit cracking of jujube is generally a serious problem,and the crack-resistance of different varieties is obviously different.V2 is a fission-resistant variation line of Muzao.The transcriptional library related to crack resistance was constructed by RNA-seq technology with the uncracked fruit of Muzao,the uncracked fruit of V2 and the cracked fruit of Muzao in fruit brittle ripening stage as test materials.The results showed that a total of 69.6 G of original data was obtained,and Q30 reached more than 92.7％with the average GC content of 45.1％.The alignment efficiency of reads between each sample and reference genome ranged from 79.82％to 85.81％by RNA-seq.Fifty differentially expressed genes were screened between the uncracked fruit of Muzao and the uncracked fruit of V2,among which 26 genes were up-regulated and 24 genes were down-regulated in V2.Twelve differentially expressed genes were screened between uncracked and cracked fruits of Muzao,among which 8 genes were up-regulated and 4 genes were down-regulated in cracked fruit.GO analysis showed that 38 of 50 and 10 of 12 differentially expressed genes between uncracked fruit of Muzao and uncracked fruit of V2,between uncracked and cracked fruits of Muzao,respectively,were functionally annotated.The biological process played an important role in crack resistance of cultivars.KEGG enrichment analysis showed that most of differentially expressed genes were distributed in metabolism-related pathways,especially starch and sucrose metabolism,purine and pyrimidine metabolism,and a few genes were classified in genetic information processing,and none of them were classified in cell processing and environmental information processing.RT-qPCR showed that the PHD finger protein gene,cellulose synthase-like protein D5 gene,protein activity of BC1 complex kinase gene and Sweet-like protein 1b gene were significantly differentially expressed,and could be used for subsequent gene functional validation studies.
万方数据
维普
