Toxicity and field application of spinetoram on Drosophila melanogaster
Drosophila melanogaster is a worldwide quarantine pest,which seriously threatens blueberry pro-duction in China.There is no effective pesticide for field prevention and control.In this study,the indoor acute toxicity of spinetoram in different concentrations to D.melanogaster was tested.The different concentrations of spinetoram suspensions were used for the control of D.melanogaster in the field,and the changes of efficacy and pesticide residues within 7 days were recorded.The most suitable concentration of spinetoram suspension was also used to carry out the Smurf test on male and female D.melanogaster.The results indicated that a 100%mortality rate was achieved on adult D.melanogaster by 2,000-,2,500-,and 3,000-fold diluted suspen-sions of 60 g/L spinetoram within 24 h of their application,among which the 2,000-and 2,500-fold dilutions had similar toxicity to D.melanogaster adults.The male insects were more sensitive to the pesticide than fe-male insects.The efficacies of spraying 2,000-and 2,500-fold dilutions of 60 g/L spinetoram in the field were similar,and the field control efficacy increased from 41.67%to 94.74%in the first 5 days after spraying 2,500-fold dilution,and the values of pesticide residue was 0.06-0.07 mg/kg in blueberries.The efficacy de-creased to 89.47%at the seventh day,and the residue value gradually decreased to 0.04 mg/kg at the same time.In addition,when fed D.melanogaster with a non-metabolizable blue dye solution,both male and female fruit flies turned blue,which was the first to demonstrate that spinetoram suspension caused intestinal damage to D.melanogaster.
Drosophila melanogasterspinetoramtoxic effectfield control efficiencypesticide residue