实验性大鼠单侧睾丸扭转术后双侧睾丸生精功能的评估
Spermatogenesis function evaluation of bilateral testes in experimental rats after unilateral testicular torsion operation
李陆义 1朱文平 1王松松 1刘子明1
作者信息
- 1. 厦门大学附属中山医院泌尿外科(福建厦门 361004)
- 折叠
摘要
目的 评估大鼠单侧睾丸扭转术后双侧睾丸生精功能.方法 雄性SD大鼠32只,随机分成4组,第1组为假手术组;第2组为2 h扭转/复位组;第3组为12 h扭转/复位组;第4组为12 h扭转/切除组,建立不同时间段720°单侧睾丸扭转动物模型.术后30 d留取双侧睾丸称重.应用流式细胞术(FCM)检测各组生精细胞凋亡和各群生精细胞百分比.结果 随着扭转时间延长,术后第2、3组患侧睾丸重量明显下降,睾丸组织正常细胞群百分比降低,早/晚期凋亡细胞群和坏死细胞群百分比增高,与第1组相比,其差异均有显著性(P＜0.05);第2组对侧睾丸重量、正常细胞群百分比与第1组相比无显著差异(P＞0.05),但早/晚期凋亡细胞群、坏死细胞群百分比与第1组相比有明显差异(P＜0.05),呈现损伤后修复过程表现;第3组对侧睾丸重量下降,与第1组相比,差异有显著性(P＜0.05);第3、4组对侧睾丸组织中正常细胞群、早/晚期凋亡细胞群、坏死细胞群百分比与第1组相比有明显差异(P＜0.05);第4组切除患侧睾丸后,对侧睾丸组织中正常细胞群、早/晚期凋亡细胞群、坏死细胞群百分比与第3组相比有明显差异(P＜0.05).结论 短时间(2 h)单侧睾丸扭转/手术复位后对两侧睾丸生精功能均有损伤,该损伤可能持续较长时间,但两侧睾丸生精功能特别是对侧可逐渐恢复,手术复位能够带来更大益处;长时间(12 h)单侧睾丸扭转/手术复位后对两侧睾丸生精功能可能产生难以恢复的损伤;如果长时间扭转导致患侧睾丸血供不能恢复,应及时切除患侧睾丸以保护对侧睾丸生精功能.
Abstract
Objective To evaluate the spermatogenesis functions of bilateral testes in rats after unilateral testicular torsion operation.Methods Total of 32 male Sprague-Dawley rats were randomly divided into four groups,and 8 rats in each group.For the rats in Group 1:a sham operation to the left testis was performed.For the rats in Group 2 and Group 3:I/R injury was created by rotating the left testis 720° in a clockwise direction for 2 h or 12 h then detorsion.For the rats in Group 4:the left testis rotated 720° for 12 h was performed through incision respectively.On 30 days after operation,the bilateral testes were collected and weighted,and their normal and apoptotic cells proportion were analyzed by the flow cytometry(FCM).Results Torsion/detorsion can significantly decreased the weight of ipsilateral testis and the percentage of normal cells,but increased the percentage of apoptotic cells and necrotic cells in Group 2 and Group 3.There were significant differences in the above indicators between Group 1 and Group 2/3(P＜0.05).There were no significant differences in the weight or the percentage of normal cells of contralateral testis between Group 2 and Group 1(P＞0.05),but significant differences in the percentage of apoptotic cells and necrotic cells(P＜0.05).The weight of contralateral testis was lower in Group 3 than that in Group 1(P＜0.05).There were also significant changes in the percentage of normal cells,apoptotic cells and necrotic cells between Group 3/4 and Group 1(P＜0.05).Compared with Group 3,the orchiectomy treatment in Group 4 increased the percentage of normal cells and decreased the percentage of apoptotic cells and necrotic cells(P＜0.05).Conclusion The short-time(2 h)testicular torsion/detorsion induce a continuous spermatogenesis damage for bilateral testis,but the spermatogenesis functions of bilateral testis,especially contralateral testis,can be gradually recovered.Therefore,surgical detorsion can provide even greater benefits.The long-time(12 h)testicular torsion/detorsion perhaps cause an unrecoverable injury to the bilateral testis.Whenever the torsional testis necrosis occurs definitely,the orchiectomy has beneficial effects against contralateral testis injury probably.
关键词
睾丸扭转/生精功能/凋亡/流式细胞术/大鼠Key words
testicular torsion/spermatogenesis/apoptosis/flow cytometry/rat引用本文复制引用
基金项目
福建省厦门市科技惠民计划(3502Z20164014)
出版年
2024
NETL
NSTL
万方数据