Objective To explore the correlation between seminal plasma oxidative stress and reproductive hormone levels with teratospermia and ICSI outcome.Methods A total of 206 male infertility patients from the reproductive medicine center of our hospital from August 2021 to December 2022 were selected as the research objects.They were grouped according to the fifth edition of the World Health Organization's Handbook on Human Semen Testing and Processing Laboratories,of which 120 patients with percentage of normal sperm morphology＜4％were included in the teratospermia group.86 cases with percentage of normal sperm morphology ≥4％were included in the normal group.The levels of seminal plasma oxidative stress(reactive oxygen species(ROS),superoxide dismutase(SOD))and reproductive hormones(follicle-stimulating hormone(FSH),luteinizing hormone(LH),testosterone(T))were detected in both groups.The influencing factors of teratospermia were analyzed.The ICSI rates of patients with different spermatozoa oxidative stress and reproductive hormone levels were compared in the teratospermia group.The correlation between the severity of teratozoospermia and seminal oxidative stress and reproductive hormone levels was analyzed.Results The proportions of advanced age,higher body mass index,smoking,drinking,varicocele and diabetes in the teratospermia group were higher than those in the control group,while sperm activity rate and forward movement sperm rate were lower than those in the control group(P＜0.05).Levels of ROS,FSH and LH in spermatozoa group were higher than those in the control group,while the levels of SOD and T in the spermatozoa group were lower than those in the control group(P＜0.05).Smoking,varicocele and ROS were independent risk factors for teratospermia,while SOD and T were independent protective factors for teratospermia(P＜0.05).In the teratospermia group,the fertilization rate was decreased with the increase of ROS level,and it was increased with the increase of seminal plasma SOD and T level(P＜0.05);Spearman's correlation analysis showed a positive correlation between the severity of teratospermia and the level of seminal plasma ROS(r=0.832,P＜0.001).Furthermore,a negative correlation was observed between the severity of teratospermia and the level of SOD(r=-0.825,P＜0.001).However,there was little correlation between the severity of teratospermia and the level of T(r=0.206,P=0.024).Conclusion Oxidative stress and abnormal expression of reproductive hormones are closely related to the occurrence of teratospermia and affect the outcome of ICSI.
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