Histopathological and transcriptomic characteristics of male genital lichen sclerosus associated urethral stricture
Objective To investigate the inflammatory characteristics of urethral strictures associated with penile lichen sclerosus(MGLS-US)through histopathology and explore molecular changes and immune cell infiltration using transcriptome sequencing.Methods Clinical characteristics and inflammatory features of urethral strictures in 24 patients with MGLS-US and 22 patients with non-male genital lichen sclerosus-related anterior urethral strictures(AUS)were assessed.Transcriptome sequencing was performed on urethral stricture tissues from 5 MGLS-US patients and 5 AUS patients to further explore the biological processes,signaling pathways,and immune cell infiltration changes in MGLS-US.Results Histopathological examination revealed a significant association between MGLS-US and severe inflammatory features,and between AUS and mild inflammatory features.Transcriptome sequencing analysis of MGLS-US indicated upregulation of biological processes such as innate immunity,adaptive immunity,T cell activation,and cell killing,as well as upregulation of immune pathways related to cytokines,cytokine action,interferon,and others.Immune cells filtration analysis showed a higher proportion of M1 macrophages,CD8 T cells,and follicular helper T cells in MGLS-US.Conclusion Urethral strictures caused by male genital lichen sclerosus exhibit more pronounced chronic inflammatory characteristics compared to those caused by non-male genital lichen sclerosus.Their transcriptome features include activation of T lymphocytes,cell killing functions,interferon pathways,and cytokine interactions.
male genital lichen sclerosusurethral strictureRNA-seqhistopathology
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