Changes in human sperm motility over time at different temperatures
Objective To understand the changes in human sperm motility over time at different temperatures for optimizing the preservation conditions of sperm in vitro.Methods Thirty-nine normal semen samples were evenly divided into two parts and placed in a water bath at 26 ℃ and 37 ℃,respectively.Their sperm motility,the proportion of forward motility,non-forward motility,and immotile sperm were measured at 0 h,2 h,4 h,and 24 h,respectively and semen samples at 0 and 24 hours were sent for bacterial culture.Results Sperm motility was 55.68％(IQR,46.06％-64.93％)in 39 semen samples at 0 h,and decreased to 37.04％(IQR,30.35％-48.40％)and 44.69％(IQR,33.10％-55.42％)after being placed at 26 ℃ and 37 ℃ for 2 h(P＜0.05).Sperm motility at 26 ℃ for 24 h was 10.39％(IQR,4.39％-17.62％),while sperm motility at 37 ℃ for 24 h was 0％(P＜0.000 1).The proportion of forward motile sperm after being at 37 ℃ and 26 ℃ for 2 hours was 33.26％(IQR,23.92％-44.58％)and 28.67％(IQR,19.41％-35.07％),respectively(P＜0.05).After 24 h,the proportions of forward motile sperm and non-forward motile sperm at 37 ℃ were 0％,while the proportions of forward motile sperm and non-forward motile sperm at 26 ℃ were 3.57％(IQR,1.65％-8.06％)and5.88％(IQR,2.19％-10.07％),respectively(P＜0.000 1).The proportion of immobile sperm(55.31％(IQR,44.42％-66.56％))at 37 ℃ after 2 h was lower than that at 26 ℃(61.99％(50.80％-69.24％))(P＜0.05).After 24 hours,the proportion of immobile sperm at 37 ℃ reached 100％,while that at 26 ℃ was 89.51％(IQR,81.78％-95.44％)(P＜0.000 1).After 48 hours bacterial culture,a lot of bacillus cereus were identified in semen samples collected at 24 hours while no bacterial was found in semen samples collected at 0 hour.Conclusions Sperm motility in vitro at 37 ℃was better than that at 26 ℃ for the first 4 hours,while sperm motility at 26 ℃ was better than that at 37 ℃ for 24 hours.The decrease in sperm motility may be related to bacterial growth and reproduction.
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