Effect of endoplasmic reticulum associated protein VAPA on acrosome biogenesis in mice
Objective To investigate the effect of endoplasmic reticulum associated protein VAPA on spermiogenesis in mice.Methods A conditional knockout mouse model with specific Vapa gene deletion in germ cells(Vapa CKO)was constructed using the loxP-cre strategy.Testes collected from wild-type and Vapa CKO adult male mice were weighed for statistical analysis.HE staining was performed to analyze the histological morphology of the seminiferous epithelium.Immunofluorescence staining and transmission electron microscopy were used to determine acrosome biogenesis in wild-type and Vapa CKO adult male mice.Results The weight of testes from Vapa CKO adult male mice exhibited a significant decrease(P<0.000 1).Specifically,spermatogenesis was arrested at round spermatids in Vapa CKO mice because round spermatids were unable to generate acrosome granule.Conclusion VAPA is involved in mouse spermiogenesis and plays an important role in acrosome biogenesis.
Vesicle-associated membrane protein-Associated Protein Aspermatogenesisacrosome biogenesisinfertilitymalemice
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