CFTR may influence sperm apoptosis and vitality via the AMPK signaling pathway
Objective To investigate the mechanism by which the cystic fibrosis transmembrane conductance regulator(CFTR)in human sperm influences sperm vitality through the AMPK signaling pathway.Methods Normal-vitality sperm and sperm with asthenozoospermia were treated with CFTRinh-172 and forskolin(FSK),respectively.Parameters including sperm vitality,acrosome enzyme activity,sperm apoptosis level,intracellular ATP,ROS,mitochondrial membrane potential(MMP),and the expression levels of CFTR,AMPK,Bax,and Bcl-2 were examined.Results CFTRinh-172 inhibited sperm vitality,acrosome enzyme activity,ATP,MMP,CFTR,and Bcl-2 expressions,also promoted sperm apoptosis rate,AMPK,and Bax expression.On the contrary,FSK treatment suppress sperm vitality,MMP,sperm apoptosis rate,AMPK,and Bax expressions,but elevated sperm acrosome enzyme activity,ATP,CFTR,and Bcl-2 levels.Conclusion CFTR influences sperm mitochondrial structure and function through the AMPK signaling pathway,and regulat sperm apoptosis and vitality.
NETL
NSTL
万方数据
