Identification and significance of a novel human sperm specific epitope peptide
Objective To screen and identify new human sperm specific antigen epitopes from candidate sperm-specific antigen epitope peptides using phage display random peptide library technology and bioinformatics methods.Methods Candidate sperm-specific epitope peptide monomers and trimers were synthesized by solid phase polypeptide synthesis.Dot immunoblotting and enzyme-linked immunosorbent assay(ELISA)were used to detect the immune reaction of the peptides with anti-sperm antibody(ASA)positive serum.ELISA was used to detect antibody titers against candidate sperm-specific epitope peptides in serum of immunized mice.The effects of the sera of the mice immunized with the candidate sperm-specific epitope peptide on human spermatozoa were examined by computer aided sperm analysis.Results High performance liquid chromatography and mass spectrometry showed that the purity and structure of the synthesized candidate sperm-specific epitope peptide were in accordance with the design.Blot and ELISA results indicated that the sperm-specific epitope peptide with the amino acid sequence n-TRRIHRHMTIRR-c and its trimers displayed strong immune response to 17 of 35 ASA positive sera.The serum from mice immunized with the peptide and its trimers significantly attenuated the sperm motility parameters compared with serum from the control group(P<0.05),with trimeric-induced decrease in motility parameters being particularly significant.(P<0.01).Conclusion The dodecapeptide with amino acid sequence n-TRRIHRHMTIRR-c is a novel human sperm-specific epitope peptide.
NETL
NSTL
万方数据
