Sperm recovery rate in different semen optimization methods and its effect on pregnancy outcome
Objective To investigate the difference of semen parameters and sperm recovery rate between density gradient centrifugation and upstream method in intrauterine insemination(IUI)and its effect on pregnancy outcome.Methods Semen samples from patients with normal semen and patients with oligospermia were randomly divided into two groups,respectively.Semen samples in one group were treated by density gradient centrifugation,and semen samples in the other group treated by upstream method.Results For the patients with normal semen parameters,their sperm concentration(53.68±16.31)106/mL,PR(85.10±7.83)％,sperm recovery rate(13.67±6.86)％and forward motility sperm recovery rate(30.35±15.80)％after density gradient centrifugation treatment were all significantly higher than those after upstream centrifugation treatment(49.85±15.25)× 106/mL;(82.35±9.52)％;(11.88±4.92)％;(25.43±11.05)％,and the differences were statistically significant.However,their clinical pregnancy rate and the live birth rate after upstream method treatment were higher than those after density gradient centrifugation treatment.For the patients with oligospermia,their sperm PR(74.26±13.11)％,sperm recovery rate(15.88±7.57)％and forward motility sperm recovery rate(46.70±29.47)％after density centrifuge method treatment were all higher than those after upstream method treatment(67.34±14.73)％;(10.50±4.58)％;(35.49±21.05)％,and the differences were statistically significant.However,their pregnancy rate and the live birth rate after upstream method treatment were higher than those after density gradient centrifugation treatment.Binary Logistic regression analysis of influencing factors for artificial insemination showed that the sperm PR after optimized and the 20 × 106≤TPMC＜30 ×106 were independently predictor for the outcome of pregnancy(P＜0.05).Conclusion The sperm recovery rate and the forward movement sperm recovery rate of density gradient centrifugation method are significantly higher than those of the upstream method,but they are not the predictors for clinical pregnancy outcome.So,the appropriate semen optimization method should be selected based on the actual situation and the semen situation of patients.
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