Therapeutical mechanism explore of Canrongzhutian Capsule for erectile dysfunction based on network pharmacology and molecular docking
Objective To study the therapeutic characteristics of Canrongzhutian Capsule on erectile dysfunction(ED)through network pharmacology and molecular docking technology.Methods The chemical constituents and corresponding target proteins in the Canrongzhutian Capsule were collected by TCMSP.Search ED disease targets of ED in GeneCards.Venny 2.1 was used to intersect the active ingredients and disease targets.The interaction network of intersecting target-active ingredient protein was constructed by Cytoscape 3.9.1.Metascape was used to analyze the intersection targets and enrich them with KEGG.Finally,Pymol and Autodock tool were used for molecular docking to verify the previous results of network pharmacology.Results Eleven kinds of traditional Chinese medicine components and their corresponding target proteins were collected in TCMSP.A total of 1 973 disease targets were retrieved by GeneCards,and 161 intersecting targets were obtained by Venny 2.1.The PPI network of active ingredient-intersection target was established by Cytoscape 3.9.1.The results of molecular docking show that the active ingredients have good binding ability with disease targets,which shows that the results of network pharmacology are reliable.Conclusion The results show that various active components in the natural capsule of Canrongzhutian can play a therapeutic role on ED through multiple channels and targets.
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