Association between sperm mitochondrial membrane potential and in vitro fertilization-embryo transfer outcomes
Objective To explore the relationship between sperm mitochondrial membrane potential (MMP) and embryo development potential,as well as pregnancy outcome in IVF/ICSI cycle.Methods The clinical data of 266 IVF cycles and 117 ICSI cycles in the reproductive center of our hospital were retrospectively analyzed.The relationship between sperm MMP and sperm concentration,sperm forward motility,sperm DNA fragmentation index ( DFI) was analyzed.Embryo development and pregnancy outcomes between MMP groups were compared in IVF/ICSI cycle.Results Sperm MMP was positively correlated with sperm concentration (r=0.216,P<0.001),sperm progressive motility (r=0.201,P<0.001),sperm normal morphology rate (r=0.122,P<0.05),and negatively correlated with sperm DFI (r=-0.175,P<0.05).In IVF cycle,embryo development had no significant difference between the MMP groups.In ICSI cycle,the transferable embryos rate (36.4％ vs 46.2％,P<0.05) and high-quality embryos rate (12.5％ vs 25.9％,P<0.001) in the low-MMP group were significantly lower than those in the high-MMP group.In addition,during IVF/ICSI cycles,the clinical pregnancy rate (58.8％ vs 59.8％;52.0％ vs 57.7％,P>0.05) and planting rate (43.1％ vs 49.6％;36.5％ vs 41.3％,P>0.05) in the low-MMP group were lower than those in the high-MMP group,but there were no statistical difference.ROC analysis results showed that the area under the curve ( AUC) of MMP for predicting the formation of high-quality embryos in ICSI cycle was 0.610,with statistical significance (P<0.05).Conclusion Level of sperm MMP is associated with sperm motility,sperm abnormalities,and reduced sperm DFI.Furthermore,MMP level affects the development of high-quality embryos in ICSI cycles,but has little effect on pregnancy outcomes in IVF/ICSI cycles.
spermatozoamembrane potentialmitochondrialfertilization in vitroembryonic developmentpregnancy outcome
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