The clinical study of Yusi-Shengjing-Fang for treatment of asthenospermia
Objective To observe the clinical efficacy of Yusi-Shengjing-Fang in the treatment of asthenospermia with spleen-kidney yang deficiency,and explore its possible molecular mechanism.Methods A total of 200 asthenospermia patients with spleen-kidney-yang deficiency in our Hospital from July 2022 to August 2023 were enrolled in the study and they were divided into two groups.The patients in the control group were administrated with Wuzi-Yanzong-Fang,and the patients in the experimental group with Yusi-Shengjing-Fang.Semen quality,TCM syndrome score and clinical efficacy of the two groups were comparatively analyzed before and after treatment.In addition,the mRNA expression levels of GPR39,MTF,MT1G,ANO1,ZIP13 and CFTR in sperm of the two groups before and after treatment were detected by qRT-PCR.Results There were no significant difference in age (31.48±5.94) years vs.(32.39±6.51) years and course of disease (2.88±2.64)years vs.(3.27±2.94)years) between the two groups (P>0.05).The percentage of sperm forward motility after treatment was significantly higher than that before treatment (28.16±15.95)％ vs.(18.47±9.01)％;(28.21±14.80)％ vs.(20.90±11.42)％,(P<0.001),and the sperm malformation index was prominently lower in both groups thanthatbeforetreatment(1.29±0.14)vs.(2.45±0.92);(1.31±0.17)vs.(2.71±0.95),(P<0.001).TheTCM syndrome scores of both groups were all significantly lower after treatment than those before treatment (2.50±1.94) points vs.(3.31±2.33) points;(3.12±2.58) pointsvs.(4.06±3.02) points,(P<0.001).ThecurativerateofYusi-Shengjing-Fang was better than that of Wuzi-Yanzong-Fang (72.31％ vs.68.69％).Additionally,qRT-PCR results showed that the mRNA expression levels of GPR39,MTF,MT1G,ANO1,ZIP13 and CFTR after treatment of Wuzi-Yanzong-Fang and Yusi-Shengjing-Fang were all obviously higher than those before treatment (P<0.05).Conclusion Yusi-Shengjing-Fang could significantly improve semen quality,and its clinical efficacy was superior to that of Wuzi-Yanzong-Fang,and no obvious adverse reactions occur.These findings indicate that Yusi-Shengjing-Fang is safe and effective in the treatment of spleen-kidney-yang deficiency type asthenospermia,worthy of clinical promotion and further study.
Yusi-Shengjing-Fangsyndrome of yang deficiency of spleen and kidneyasthenozoospermiaclinical study
NETL
NSTL
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