首页|跨物种扩增筛选获得蓝马鸡(Crossoptilon auritum)微卫星多态分子标记

跨物种扩增筛选获得蓝马鸡(Crossoptilon auritum)微卫星多态分子标记

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微卫星是分子生态学研究常用分子标记之一.与建库筛选获得微卫星的方法相比,跨物种扩增是快速获得近缘物种微卫星分子标记的一种有效方法.本研究通过对中国特有雉类蓝马鸡(Crossoptilon auritum)进行跨物种扩增,从鸡形目112个微卫星位点筛选获得了11个微卫星多态分子标记.这组分子标记的多态性从中度到高度,并包含一个Z-染色体连锁位点.利用这组分子标记对已知谱系的笼养个体进行初步亲子鉴定,表明这组微卫星分子标记的高度实用性能够为马鸡属及鸡形目其他濒危物种的保护遗传学研究提供有力工具.
A panel of polymorphic microsatellites in the Blue Eared Pheasant (Crossoptilon auritum) developed by cross-species amplification
Polymorphic microsatellites are among the versatile genetic markers in molecular ecology studies.In contrast to de novo isolation of microsatellites from target species,cross-species amplification is a cost-effective approach for a fast development of microsatellite markers from closely related taxa.In our study,we cross-amplified a panel of polymorphic microsatellite markers for the Blue Eared Pheasant (Crossoptilon auritum),a species endemic to China of considerable conservation concern.We obtained 11 polymorphic microsatellite markers selected from 112 candidate loci,originally isolated from other Galliforme species.This panel of makers has shown moderate to high levels of polymorphism and include a Z-chromosomal linkage locus.We carried out preliminary analyses of parentage among captive individuals with a known pedigree using this new panel of microsatellites.Our results suggest that the high utility of these markers may be powerful tools for studies in conservation genetics of eared-pheasants and other endangered Galliforme species.

Crossoptilon auritummicrosatellitescross-species amplificationZ-chromosomal linkage

谷浪屿、刘阳、王宁、张正旺

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北京师范大学生命科学学院,生物多样性与生态工程教育部重点实验室,北京,100875

瑞士伯尔尼大学生态与进化研究所进化生态计算和分子群体遗传学组,伯尔尼,3012

中山大学生命科学学院,有害生物控制与资源利用国家重点实验室,广州,510275

Crossoptilon auritum 微卫星 跨物种扩增 Z-染色体连锁

study was supported by the National Key Project of the Scientific and Technical Supporting Programs Funded by the Ministry o国家自然科学基金

2012BAC01B0630570234

2012

10.5122/cbirds.2012.0010

鸟类学研究(英文版)
北京林业大学

鸟类学研究(英文版)

CSCD
影响因子：0.553
ISSN：2055-6187
年,卷(期)：2012.3(2)
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