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DDRT-PCR Analysis of Wheat Roots Under Iron-Deficient Condition and Differential Expression of ABC Gene

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Differential expression of gene in iron-efficient wheat cultivar Jing411 and iron-inefficient cul-tivar Sanshumai3 under iron-deficiency and iron-sufficiency conditions was revealed by differential display re-verse transcript PCR (DDRT-PCR) method. Northern blotting was carried out using ATP-binding transporter(ABC) cDNA obtained from DDRT-PCR products of the cuitivar Jing411 as probe. Our results suggested thatABC gene expression was suppressed under iron-deficiency condition.

WheatRootsIron nutritionDDRT-PCRGene expression

YIN Li-ping、LIU Wei-zhong、LIU Xiang-lin、HUANG Qin-ni、ZHANG Fu-suo

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Department of Biology, Capital Normal University, Beijing 100037, P.R.China

China Agricultural University, Beijing 100094, P.R.China

2002

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

影响因子:0.576
ISSN:2095-3119
年,卷(期):2002.1(12)
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