首页|Isolation and Identification of Virus dsRNA from Strawberry Plants
Isolation and Identification of Virus dsRNA from Strawberry Plants
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国家科技期刊平台
NETL
NSTL
万方数据
维普
The analysis of virus genome is based on nucleic acid isolation. The aims of this study were to develop a method for isolation and identification of virus double-stranded ribonucleic acid (dsRNA) and to elucidate the nucleotide sequences of strawberry virus. Using the modified method, virus dsRNA was extracted from strawberry virus indicator plants and cultivated strawberry plants and detected using agarose gel electrophoresis with ethidium bromide staining and reverse transcription-polymerase chain reaction (RT-PCR). The quantity of virus dsRNA varied among strawberry cultivars. The quantity of dsRNA from in vitro plantlets was higher than that from the young leaves of field plants. For the field-grown plants, there was more dsRNA in the young leaves. Virus dsRNA extracted from strawberry plants was resistant to deoxyribonuclease Ⅰ (DNase Ⅰ ), but evidently, it became resistant to ribonuclease A (RNase A) only in the presence of 0.5 M NaCl. Its bands in agarose gel could be readily recycled using an agarose gel DNA purification kit. With RT-PCR, the segments of both strawberry mottle virus and Strawberry mild yellow edge virus genomes were amplified by using the virus dsRNA recycled from gel or treated with DNase Ⅰ /RNase A as templates. The system developed for dsRNA isolation and identification in strawberry plants laid a sound foundation for the work on genome analysis of strawberry virus isolates in China.
dsRNAvirusstrawberryisolationRT-PCR
LI He、DAI Hong-yan、ZHANG Zhi-hong、GAO Xiu-yan、DU Guo-dong、ZHANG Xin-yu
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College of Horticulture, Shenyang Agricultural University, Shenyang 110161, P.R. China
国家自然科学基金Scientific Research Start-up Foundation of Ministry of Education for Returned Overseas Students, China
国家科技期刊平台
NSTL
万方数据
维普
