首页|Characterization of the Purified Glutathione S-transferases from Two Psocids Liposcelis bostrychophila and L.entomophila

Characterization of the Purified Glutathione S-transferases from Two Psocids Liposcelis bostrychophila and L.entomophila

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Glutathione S-transferases(GSTs)from Liposcelis bostrychophila Badonnel and L.entomophila(Enderlein)(Psocoptera:Liposcelididae)were purified by glutathione-agarose affinity chromatography,and characterized subsequently by their Michaelis-Menten kinetics toward the artificial substrates 1-chloro-2,4-dinitrobenzene(CDNB)and reduced glutathione(GSH),respectively.The specific activity of the purified GST toward CDNB was 2.3-fold higher in L.bostrychophila than in L.entomophila.Though the specific activities of purified enzymes varied between the two species,the purification yields were similar.SDS-PAGE revealed one band at 23 kDa for both the species.GSTs of L.entomophila exhibited higher Michaelis-Menten constants(Km)but lower maximal velocity(Vmax)values than those of L.bostrychophila.The optimum pH for CDNB conjugation of L.bostrychophila and L.entomophila GSTs was 7.0 and 7.5,and optimum temperature was35 and 40℃,respectively.Inhibition kinetics showed that cibacron blue,curcumin,bromosulfalein,ethacrynic acid,and carbosulfan had excellent inhibitory effects on GSTs in both species,but the inhibitory effects of beta-cypermethrin,fenpropathrin,tetraethylthiuram disulfide,and diethyl maleate were not significant.

GSTspurificationpsocidsxenobiotic compounds

DOU Wei、XIAO Li-sha、NIU Jin-zhi、JIANG Hong-bo、WANG Jin-jun

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Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University,Chongqing 400716,P.R.China

This research was funded in part by the National Natural Sciences Foundation of ChinaSpecialized Research Fund for the Doctoral Program of Higher Education of China to Prof. Wang JinjunDoctoral Program of Southwest University of ChinaFundamental Research Funds for the Central Universities, China

30871631200806350009SWU109023XDJK2009C112

2010

10.1016/S1671-2927(09)60184-X

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2010.9(7)
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