首页|Functional Characterization of a NEM1-Like Gene in Magnaporthe oryzae

Functional Characterization of a NEM1-Like Gene in Magnaporthe oryzae

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  • 国家科技期刊平台
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  • NSTL
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Magnaporthe oryzae,a filamentous ascomycete fungus,is well known as the causal agent of rice blast.With the technology of suppression subtractive hybridization (SSH),it was previously found that MGG_06001 (or named MoNEM1),a gene of M.oryzae homologous to the NEM1 (nuclear envelope morphology protein 1) gene of baker's yeast (Saccharomyces cerevisiae),is differentially expressed between the mature appressium and the conidium and mycelium.This study aimed to characterize the function of MoNEM1 gene by knocking it out using the method of target gene replacement.The △Moneml mutants exhibited reduced mycelial growth and conidiation.However,disruption of MoNEM1 gene does not affect the pathogenicity of M.oryzae on barley and rice.

Magnaporthe oryzaeMoNEM1conidiationgrowthpathogenicity

WANG Ying、JIAO Tian-lei、LIU Xiao-hong、LIN Fu-chen、WU Wei-ren

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Department of Agronomy, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310029, P.R.China

Institute of Biotechnology, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310029, P.R.China

School of Life Sciences, Fujian Agriculture & Forestry University, Fuzhou 350002, P.R. China

国家自然科学基金国家自然科学基金

3067112331000077

2011

10.1016/S1671-2927(11)60131-4

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2011.10(9)
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