首页|A Modified Method for the Development of SSR Molecular Markers Based on Redundant EST Data and Its Application in Soybean

A Modified Method for the Development of SSR Molecular Markers Based on Redundant EST Data and Its Application in Soybean

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
EST-derived SSR marker has been developed in many species,but few methods of high efficiency have been reported for the exploitation of EST-SSR markers.Thus,a high efficiency method for mining millions of redundant EST data is needed.A modified method for the EST-SSR development with high efficiency was established based on the redundant EST data of soybean in this study.The method achieved its function through classifying ESTs according to the same SSR motif and detected candidate loci with redundant sequences.In this study,a total of 80 polymorphic EST-SSR markers of soybean were developed,50 of them were exploited by this modified method which proved the higher speed and efficiency of this method.All the 80 polymorphic EST-SSRs were mapped on soybean physical map through in silico mapping and 15 markers were integrated on a genetic map constructed in previous study.A software named hpSSR (high polymorphic SSR) was programmed based on the concept of the up-built method for EST-SSR development.This method is not only pragmatic for EST-SSR exploitation in soybean,but also effective for the development of the marker in other species if the redundancy EST data is available.

EST-SSRsoybeanpolymorphismgenetic mappingin silico mapping

ZHAO Xue、CHANG Wei、HAN Ying-peng、TENG Wei-li、LI Wen-bin

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Key Laboratory of Soybean Biology, Ministry of Education/Soybean Research Institute, Northeast Agricultural University, Harbin 150030,P.R. China

Key Laboratory of Soybean Biology,Ministry of Education,ChinaSoybean Development Centre,Ministry of Agriculture,ChinaNational High-Tech R&D Program of ChinaNational Core Soybean Genetic Engineering Project,ChinaNational Core Soybean Genetic Engineering Project,ChinaNational Core Soybean Genetic Engineering Project,China国家自然科学基金国家自然科学基金国家重点基础研究发展规划(973计划)Soybean Molecular Design Team of Education of Ministry,ChinaSoybean Molecular Design Team of the Heilongjiang Provincial Education Department,ChinaTechnology Project of Harbin,China

2006AA10Z1F12008ZX08004-0022009ZX08004-002B2009ZX08009-089B60932008309718102009CB118400115410252009RFQXN085

2012

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSCDSCI
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2012.11(4)
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