首页|Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.)

Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.)

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  • 国家科技期刊平台
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Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cu-cumber, and its frequency has been up to 23%. For example, signiifcantly enhancement of the transformation efifciency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efifcient positive selection system in lots of experiments. This review is to summarize some key factors in-lfuencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efifciency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.

Cucumis sativus L.plant regenerationgenetic transformationpositive selection system

WANG Shun-li、Seong Sub Ku、YE Xing-guo、HE Cong-fen、Suk Yoon Kwon、Pil Son Choi

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Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Department of 0riental Pharmaceutical Development, Nambu University, Gwangju 506-824, Korea

Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, P.R.China

School of Sciences, Beijing Technology and Business University, Beijing 100048, P.R.China

Plant Systems Engineering Research Center, KRIBB, Daejeon 305-806, Korea

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This research was ifnancially supported by grants from the Biogreen 21 Program, RDA, KoreaAgricultural Science and Technology Innovation Program (ASTIP) of Chinese Academy of Agricultural SciencesBeijing Municipal Education Commis-sion, China

PJ008103042014-2015KM200910011001

2015

10.1016/S2095-3119(14)60899-6

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCDCSCDSCI
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2015.(3)
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