首页|iTRAQ quantitative analysis of plasma proteome changes of cow from pregnancy to lactation

iTRAQ quantitative analysis of plasma proteome changes of cow from pregnancy to lactation

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  • 国家科技期刊平台
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Dairy cows undergo tremendous changes in physiological, metabolism and the immune function from pregnancy to lac-tation that are associated with cows being susceptible to metabolic and infectious diseases. The objective of this study is to investigate the changes of plasma proteome on 21 d before expected calving and 1 d after calving from dairy cows using an integrated proteomic approach consisting of minor abundance protein enrichment by ProteoMiner beads, protein labeling by isobaric tags for relative and absolute quantiifcation, and protein identiifcation by liquid chromatography coupled with tandem mass spectrometry. Nineteen proteins were changed around the time of calving. These proteins were asso-ciated with response to stress, including acute-phase response and defense response, based on the proteins annotation. In particular, three up-regulated proteins after calving including factor V,α2-antiplasmin and prothrombin were assigned into the complement and coagulation pathway. These results may provide new information in elucidating host response to lactation and parturition stress, and inlfammatory-like conditions at the protein level. Differential proteins may serve as potential markers to regulate the lactation and parturition stress in periparturient dairy cows.

dairy cowperiparturientplasmaproteomicquantitative analysis

MA Lu、BU Deng-pan、YANG Yong-xing、YAN Su-mei、WANG Jia-qi

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Inner Mongolia Agricultural University, Huhhot 010018, P.R.China

State Key Laboratory of Animal Nutrition/CAAS-ICRAF Joint Laboratory on Agroforestry and Sustainable Animal Husbandry/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China

project was supported by the National Key Technology R&D Program of ChinaResearch Program of the State Key Laboratory of Animal Nutrition, Chinaand the Synergetic Innova-tion Center of Food Safety and Nutrition, China

2012BAD12B02-52004DA125184G1103

2015

10.1016/S2095-3119(14)60916-3

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCDCSCDSCI
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2015.(7)
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