首页|A novel Arabidopsis miRNA, ath-miR38-3P, is involved in response to Sclerotinia sclerotiorum infection
A novel Arabidopsis miRNA, ath-miR38-3P, is involved in response to Sclerotinia sclerotiorum infection
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国家科技期刊平台
NETL
NSTL
维普
万方数据
Plant defense responses against penetration or colonization of pathogens are mediated by activation and repression of a large array of genes.Host endogenous small RNAs are essential in gene expression reprogramming process.We identified a new Arabidopsis microRNA (miRNA) ath-miR38-3P by high-throughput sequencing and further confirmed it by Northern blot assay.Interestingly,ath-miR38-3P was highly induced after infection of the pathogen Sclerotinia sclerotiorum.Further analysis based on the miRNAtarget database demonstrated that ath-miR38-3P might target to five putative genes:AT2G03140,AT5G59430,AT5G66320,AT1G36620 and AT3G03820.To confirm the target,we conducted the quantitative real-time PCR to observe the expression pattern of each candidate gene.The results showed that only AT3G03820 was down-regulated after inoculation of S.sclerotiorum.In addition,overexpression of ath-miR38-3P down-regulates AT3G03820,suggesting AT3G03820 might represent the target for ath-miR38-3P.Our results may provide the useful information for further studying the biological function of a novel ath-miR38-3P and its targets in Arabidopsis-Sclerotinia interaction.
Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China
Key Laboratory of Bio-Pesticide and Chemistry Biology, Ministry of Education, Fuzhou 350002, P.R.China
grants from the Special Fund for Agro-scientific Research in the Public Interest,ChinaFujian Provincial Science Foundation,China
国家科技期刊平台
NSTL
维普
万方数据
