首页|Species-specific COI primers for rapid identification of a globally significant invasive pest, the cassava mealybug Phenacoccus manihoti Matile-Ferrero

Species-specific COI primers for rapid identification of a globally significant invasive pest, the cassava mealybug Phenacoccus manihoti Matile-Ferrero

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The globally invasive cassava mealybug Phenacoccus manihoti Matile-Ferrero is a pernicious pest of cassava,and its recent introduction into Asia has raised considerable alarm.To slow or prevent further invasion,an accurate,simple,and developmental-stage-independent detection method for P.manihoti is required.In the present study,a PCR method based on a species-specific mitochondrial DNA cytochrome oxidase I (SS-COI) marker was developed for rapid identification of P.manihoti.One pair of SS-COI primers (PMSSZW-1F and PMSSZW-1R) was designed based on sequence variations in the COI gene among P.manihoti and related mealybug species.Specificity of the primer pair was validated on 21 closely related species.Sensitivity tests were performed on four immature developmental stages and female adults.Efficacy tests demonstrated that at the relatively low concentration of (135.2±14.7) pg μL-1 resuspended DNA,the specific fragment was detected in all replicates.Furthermore,the SS-COI primer pair was assayed on three populations of P.manihoti from major exporting countries of cassava.The PCR assay was proved to be a rapid,simple,and reliable molecular measure for the identification of P.manihoti.This tool will be useful for quarantine,monitoring,and management of this invasive pest.

Phenacoccus manihoticassava mealybuginvasive pestmolecular identificationspecies-specific COI primers

WANG Yu-sheng、TIAN Hu、WAN Fang-hao、ZHANG Gui-fen

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State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory of Integrated Pest Management of Crop,Ministry of Agriculture/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China

Caofeidian Sub-Center of Hebei Entry-Exit Inspection and Quarantine Technical Center, Tangshan 063200, P.R.China

We are grateful to Ms.Guo Rong from the National Agro-Tech Extension and Service Center,for providing specimens of P.manihoti frProf.Hou Maolin from the Institute of Plant Protection,Chinese Academy of Agricultural Sciences (CAAS),for providing specimens oProf.Zhou Pei from the Wuxi Entry-Exit Inspection and Quarantine Bureau,China for providing specimens of Planococcus minor from Prof.Zhou Pei from the Wuxi Entry-Exit Inspection and Quarantine Bureau,China for providing specimens of Planococcus minor from Prof.Zhou Pei from the Wuxi Entry-Exit Inspection and Quarantine Bureau,China for providing specimens of Planococcus minor from and the Science and Technology Innovation Program of CAAS

2017YFC12006002016YFC12012002015BAD08A16caascx-2013-2018-IAS

2019

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCDCSCDSCI
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2019.18(5)
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