首页|Identification and gene mapping of the starch accumulation and premature leaf senescence mutant ossac4 in rice

Identification and gene mapping of the starch accumulation and premature leaf senescence mutant ossac4 in rice

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
The rice mutant ossac4 (starch accumulating 4) was raised from seeds of the rice (Oryza sativa L.) indica maintainer line Xinong 1B treated with ethyl methanesulfonate. The distal and medial portions of the second leaf displayed premature senescence in the ossac4 mutant at the four-leaf stage. Physiological and biochemical analysis, and cytological examination revealed that the ossac4 mutant exhibited the premature leaf senescence phenotype. At the four-leaf stage, the leaves of the ossac4 mutant exhibited significantly increased contents of starch compared with those of the wild type (WT). Quantitative real-time PCR analysis showed that the expression levels of photosynthesis-associated genes were down-regulated and the expression levels of glucose metabolism-associated genes were abnormal. Genetic analysis indicated that the ossac4 mutation was controlled by a single recessive nuclear gene. The OsSAC4 gene was localized to a 322.7-kb interval between the simple-sequence repeat marker XYH11-90 and the single-nucleotide polymorphism marker SNP5300 on chromosome 11. The target interval contained 20 annotated genes. The present results demonstrated that ossac4 represents a novel starch accumulation and premature leaf senescence mutant, and lays the foundation for cloning and functional analysis of OsSAC4.

Oryza sativa L.gene mappingglucose metabolismpremature senescencestarch accumulation

ZHU Mao-di、CHEN Xin-long、ZHU Xiao-yan、XING Ya-di、DU Dan、ZHANG Ying-ying、LIU Ming-ming、ZHANG Qiu-li、LU Xin、PENG Sha-sha、HE Guang-hua、ZHANG Tian-quan

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Rice Research Institute, Key Laboratory of Application and Safety Control of Genetically Modified Crops, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, P.R.China

This work was supported by the National Key Research and Development Program of ChinaProject of Chongqing Science & Technology Commission Grants, ChinaCSTCCXLJRC201713 and CSTC2016SHMSZTZX0017), andFundamental Research Funds for the Central Universities, China

2017YFD0100201XDJK2017C030

2020

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCDCSCDSCI
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2020.19(9)
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