首页|High-throughput screening system of citrus bacterial canker-associated transcription factors and its application to the regulation of citrus canker resistance

High-throughput screening system of citrus bacterial canker-associated transcription factors and its application to the regulation of citrus canker resistance

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  • 国家科技期刊平台
  • NETL
  • NSTL
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One of the main diseases that adversely impacts the global citrus industry is citrus bacterial canker(CBC),caused by the bacteria Xanthomonas citri subsp.citri(Xcc).Response to CBC is a complex process,with both protein-DNA as well as protein-protein interactions for the regulatory network.To detect such interactions in CBC resistant regulation,a citrus high-throughput screening system with 203 CBC-inducible transcription factors(TFs),were developed.Screening the upstream regulators of target by yeast-one hybrid(Y1H)methods was also performed.A regulatory module of CBC resistance was identified based on this system.One TF(CsDOF5.8)was explored due to its interactions with the 1-kb promoter fragment of CsPrx25,a resistant gene of CBC involved in reactive oxygen species(ROS)homeostasis regulation.Electrophoretic mobility shift assay(EMSA),dual-LUC assays,as well as transient overexpression of CsDOF5.8,further validated the interactions and transcriptional regulation.The CsDOF5.8-CsPrx25 promoter interaction revealed a complex pathway that governs the regulation of CBC resistance via H2O2 homeostasis.The high-throughput Y1H/Y2H screening system could be an efficient tool for studying regulatory pathways or network of CBC resistance regulation.In addition,it could highlight the potential of these candidate genes as targets for efforts to breed CBC-resistant citrus varieties.

citrus bacterial canker(CBC)high-throughput screening systemtranscription factor(TF)yeast-one hybrid(Y1H)CsPrx25

Jia Fu、Jie Fan、Chenxi Zhang、Yongyao Fu、Baohang Xian、Qiyuan Yu、Xin Huang、Wen Yang、Shanchun Chen、Yongrui He、Qiang Li

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Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science City,Citrus Research Institute,Southwest University,Chongqing 400712,China

School of Advanced Agriculture and Bioengineering,Yangtze Normal University,Chongqing 408100,China

National Citrus Engineering Research Center,Chongqing 400712,China

National Key Research and Development Program of Chinaearmarked fund for the China Agriculture Research SystemFundamental Research Funds for the Central Universities,ChinaScience and Technology Research Program of Chongqing Municipal Education Commission,China

2022YFD1201600CARS-26SWU-XDJH202308KJQN202001418

2024

10.1016/j.jia.2023.11.011

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2024.23(1)
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