首页|New insights into developmental biology of Eimeria tenella revealed by comparative analysis of mRNA N6-methyladenosine modification between unsporulated oocysts and sporulated oocysts

New insights into developmental biology of Eimeria tenella revealed by comparative analysis of mRNA N6-methyladenosine modification between unsporulated oocysts and sporulated oocysts

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
Evidence showed that N6-methyladenosine(m6A)modification plays a pivotal role in influencing RNA fate and is strongly associated with cell growth and developmental processes in many species.However,no information regarding m6A modification in Eimeria tenella is currently available.In the present study,we surveyed the transcriptome-wide prevalence of m6A in sporulated oocysts and unsporulated oocysts of E.tenella.Methylated RNA immunoprecipitation sequencing(MeRIP-seq)analysis showed that m6A modification was most abundant in the coding sequences,followed by stop codon.There were 3,903 hypermethylated and 3,178 hypomethylated mRNAs in sporulated oocysts compared with unsporulated oocysts.Further joint analysis suggested that m6A modification of the majority of genes was positively correlated with mRNA expression.The mRNA relative expression and m6A level of the selected genes were confirmed by quantitative reverse transcription PCR(RT-qPCR)and MeRIP-qPCR.GO and KEGG analysis indicated that differentially m6A methylated genes(DMMGs)with significant differences in mRNA expression were closely related to processes such as regulation of gene expression,epigenetic,microtubule,autophagy-other and TOR signaling.Moreover,a total of 96 DMMGs without significant differences in mRNA expression showed significant differences at protein level.GO and pathway enrichment analysis of the 96 genes showed that RNA methylation may be involved in cell biosynthesis and metabolism of E.tenella.We firstly present a map of RNA m6A modification in E.tenella,which provides significant insights into developmental biology of E.tenella.

Eimeria tenellam6ARNA methylationMeRIP-seqRNA-seqproteomic analysis

Qing Liu、Bingjin Mu、Yijing Meng、Linmei Yu、Zirui Wang、Tao Jia、Wenbin Zheng、Wenwei Gao、Shichen Xie、Xingquan Zhu

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Laboratory of Parasitic Diseases,College of Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China

Research Center for Parasites&Vectors,College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China

National Natural Science Foundation of ChinaShanxi Provincial Key Research and Development Program,ChinaFund for Shanxi"1331 Project",ChinaScience and Technology Innovation Program of Shanxi Agricultural University,ChinaSpecial Research Fund of Shanxi Agricultural University for Highlevel Talents,China

319022982022ZDYF12620211331-132017YJ102021XG001

2024

10.1016/j.jia.2023.07.011

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2024.23(1)
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