首页|Targeted mutations of BnPAP2 lead to a yellow seed coat in Brassica napus L.
Targeted mutations of BnPAP2 lead to a yellow seed coat in Brassica napus L.
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
万方数据
维普
Targeted mutations of BnPAP2 lead to a yellow seed coat in Brassica napus L.
The yellow seed trait is preferred by breeders for its potential to improve the seed quality and commercial value of Brassica napus.In the present study,we produced yellow seed mutants using a CRISPR/Cas9 system when the two BnPAP2 homologs were knocked out.Histochemical staining of the seed coat demonstrated that proanthocyanidin accumulation was significantly reduced in the pap2 double mutants and decreased specifically in the endothelial and palisade layer cells of the seed coat.Transcriptomic and metabolite profiling analysis suggested that disruption of the BnPAP2 genes could reduce the expression of structural and regulated genes in the phenylpropanoid and flavonoid biosynthetic pathways.The broad suppression of these genes might hinder proanthocyanidin accumulation during seed development,and thereby causing the yellow seed trait in B.napus.These results indicate that BnPAP2 might play a vital role in the regulatory network controlling proanthocyanidin accumulation.
Hunan Research Center of Heterosis Utilization in Rapeseed/Crop Research Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,China
Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization/School of Life and Health Science,Hunan University of Science and Technology,Xiangtan 411201,China
Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs/Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China
万方数据
维普
