Abstract
Nitrogen(N),phosphorus(P),and potassium(K)are essential macronutrients that are crucial not only for maize growth and development,but also for crop yield and quality.The genetic basis of macronutrient dynamics and accumulation during grain filling in maize remains largely unknown.In this study,we evaluated grain N,P,and K concentrations in 206 recombinant inbred lines generated from a cross of DH1M and T877 at six time points after pollination.We then calculated conditional phenotypic values at different time intervals to explore the dynamic characteristics of the N,P,and K concentrations.Abundant phenotypic variations were observed in the concentrations and net changes of these nutrients.Unconditional quantitative trait locus(QTL)mapping revealed 41 non-redundant QTLs,including 17,16,and 14 for the N,P,and K concentrations,respectively.Conditional QTL mapping uncovered 39 non-redundant QTLs related to net changes in the N,P,and K concentrations.By combining QTL,gene expression,co-expression analysis,and comparative genomic data,we identified 44,36,and 44 candidate genes for the N,P,and K concentrations,respectively,including GRMZM2G371058 encoding a Dof-type zinc finger DNA-binding family protein,which was associated with the N concentration,and GRMZM2G113967 encoding a CBL-interacting protein kinase,which was related to the K concentration.The results deepen our understanding of the genetic factors controlling N,P,and K accumulation during maize grain development and provide valuable genes for the genetic improvement of nutrient concentrations in maize.
基金项目
Seed Industry Revitalization Project of Jiangsu Province,China(JBGS[2021]009)
National Natural Science Foundation of China(32061143030)
National Natural Science Foundation of China(31972487)
Jiangsu Province University Basic Science Research Project,China(21KJA210002)
Key Research and Development Program of Jiangsu Province,China(BE2022343)
Innovative Research Team of Universities in Jiangsu Province,China()
Highend Talent Project of Yangzhou University,China()
Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),China()
Qing Lan Project of Jiangsu Province,China()
国家科技期刊平台
NSTL
万方数据